rabbit anti cd45 polyclonal antibody  (Bioss)

Journal: Nature Communications

Article Title: Dual asparagine-depriving nanoparticles against solid tumors

doi: 10.1038/s41467-025-60798-y

Figure Lengend Snippet: a In vivo fluorescence imaging of tumor-bearing mice 24 h after intravenous injection of M Cy5.5 As and M Cy5.5 AHAs ( n = 3 mice). The unit of the scale bar is ×10 9 [p/s/cm 2 /sr]/[µW/cm 2 ]. b Left: Confocal fluorescence imaging of tumor sections after administration of M Cy5.5 As and M Cy5.5 AHAs. The 4T1 tumor cells and immune cells were indicated as CD44 and CD45 positive cells (red fluorescence), respectively. M Cy5.5 As and M Cy5.5 AHAs were pseudo-colored with green fluorescence. DAPI (blue fluorescence) was used to label the nuclei. Scale bars: 15 µm. Right: Values of Pearson’s correlation coefficient between Cy5.5-incorporated NPs and 4T1 tumor cells or immune cells. Data are represented as mean ± SD ( n = 10 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. c Average tumor growth curves. Data are represented as mean ± SD ( n = 6 mice). P values were calculated using a two-way ANOVA followed by Tukey’s multiple comparisons test. d Survival curves of the mice receiving indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. e The Asn levels in tumor cells isolated from tumors on day 26 after indicated treatment. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. f Heatmap of mRNA levels of ASNS , PSAT1 , GPT2 , MTHFD2 , and SLC1A5 in tumor cells isolated from tumors after indicated treatment. g Left: representative H&E-stained sections of lungs from indicated groups. Metastatic tumors are indicated with black dotted circles. Scale bars: 100 μm. See also Supplementary Fig. for complete data. Right: the number of tumor foci in lungs after indicated treatment. Data are represented as mean ± SD ( n = 3 mice). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. h , i Expression levels of N-cadherin ( h ) and E-cadherin ( i ) in tumors after indicated treatments. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using a one-way ANOVA followed by Tukey’s post-hoc test. Asn asparagine, Cy5.5 cyanine 5.5, Rot rotenone, ASNase L -asparaginase. Source data are provided as a Source Data file.

Article Snippet: Tumor sections were stained with either anti-CD44 rabbit polyclonal antibody (Servicebio, GB112054 ) or anti-CD45 rabbit polyclonal antibody (Servicebio, GB113885 ), and visualized using CLSM.

Techniques: In Vivo, Fluorescence, Imaging, Injection, Two Tailed Test, Isolation, Staining, Expressing

Journal: Nature Communications

Article Title: Dual asparagine-depriving nanoparticles against solid tumors

doi: 10.1038/s41467-025-60798-y

Figure Lengend Snippet: a Schematic illustration of the experiment design for post-surgical therapy. Fourteen days after tumor inoculation, primary tumors were removed. Two days later, mice were subjected to various treatments every 3 days for a total of eight doses. On day 75, mice with no sign of tumor relapse and metastasis were challenged with 4T1-Luc cells. b In vivo bioluminescence imaging of the mice after indicated treatment ( n = 6 mice). The unit of the scale bar is ×10 7 p/s/cm 2 /sr. c Tumor growth curves of individual mice after indicated treatment ( n = 6 mice). d Ex vivo bioluminescence images of major organs ( n = 3 mice). The unit of the scale bar is ×10 6 p/s/cm 2 /sr. e Survival curves of the mice receiving the indicated treatment ( n = 6 mice). P values were calculated using a log-rank (Mantel–Cox) test. f In vivo bioluminescence imaging of mice before (day 74) and after (day 75, day 95, and day 105) tumor rechallenge ( n = 5 mice). The unit of the scale bar is ×10 7 p/s/cm 2 /sr. g , h FACS analysis of CD4 + Tcm (gated on CD45 + CD4 + population) ( g ) and CD8 + Tcm cells (gated on CD45 + CD8 + population) ( h ) in splenocytes. Data are represented as mean ± SD ( n = 3 independent samples). P values were calculated using an unpaired two-tailed Student’s t test. Rot rotenone, ASNase L -asparaginase, He heart, Li liver, Sp spleen, Lu lung, Ki kidney, Tcm central memory T. Source data are provided as a Source Data file.

Article Snippet: Tumor sections were stained with either anti-CD44 rabbit polyclonal antibody (Servicebio, GB112054 ) or anti-CD45 rabbit polyclonal antibody (Servicebio, GB113885 ), and visualized using CLSM.

Techniques: In Vivo, Imaging, Ex Vivo, Two Tailed Test

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Integrated multiomic analysis identifies TRIP13 as a mediator of alveolar epithelial type II cell dysfunction in idiopathic pulmonary fibrosis

doi: 10.1016/j.bbadis.2024.167572

Figure Lengend Snippet: Key resources.

Article Snippet: Rabbit IgG anti-human CD45 polyclonal, unconjugated; 1:200 , Santa Cruz Biotech , Cat# sc-25,590 RRID: AB_2174143.

Techniques: Plasmid Preparation, Magnetic Beads, Recombinant, Blocking Assay, Lysis, Protease Inhibitor, Red Blood Cell Lysis, Software, Microscopy, Fluorescence, Imaging

Journal: Frontiers in Cell and Developmental Biology

Article Title: Syndecan-1 as a predictor of vulnerable atherosclerotic plaques

doi: 10.3389/fcell.2024.1415788

Figure Lengend Snippet: Analysis of destabilizing components in atherosclerotic plaque. (A) The expression of lipid deposition in aortic root plaques was detected by oil red O with a scale bar of 100 μm, immunofluorescence detection of CD68 expression (macrophage marker) and VEGF in aortic root plaques with a scale bar of 20 μm, and IHC detection of MMP-9 expression in aortic root plaques with a scale bar of 100 μm. (B–E) Statistical chart for quantitative analysis of oil red o staining, CD68, VEGF and MMP-9 in the aortic root plaques (n = 6, one-way ANOVA). (F) Immunofluorescence detection of BODIPY and VEGF expression in LCCA plaques with a scale bar of 100 μm. IHC detection of CD68 and MMP-9 expression in LLCCA plaques with a scale bar of 100 μm. (G–J) Statistical chart for quantitative analysis of BODIPY staining, VEGF, CD68 and MMP-9 in the LCCA plaques (n = 6, unpaired t -test). * P < 0.05, ** P < 0.01, *** P < 0.001, compared with CD group; # P < 0.05, ## P < 0.01, ### P < 0.001, compared with CD + PLCA group; && P < 0.01, &&& P < 0.001, compared to HFD group.

Article Snippet: After blocking, sections were incubated with a rabbit anti-human CD68 (1:100, bs-4819R, BIOSS, China), α-SMA (1:100, A1011, Abclonal, China) and mouse anti-human VEGF (1:100, TA500289, Origene, United States of America) antibodies overnight at 4°C.

Techniques: Expressing, Immunofluorescence, Marker, Staining